Figure 6.

Amniotic fluid stem cells (AFS)-derived cells contribute stromal components for extending ducts. The 10⁵ AFSs derived from a Rosa26 donor were injected into noncleared fat pads of 3-week-old recipient mice and analyzed 2 weeks later. (a) Whole-mount analysis and tissue sections of X-Gal-stained whole mounts demonstrate the presence of LacZ⁺ cells in the stroma of extending ducts. (b) AFS-derived LacZ⁺ cells retain the expression of α-SMA but did not integrate into the myoepithelial cell layer. Scale bar for whole mount is 1,000 mm, and for tissue sections, 50 μm; (*) injection site, (Blue) X-Gal stain, (Pink) Carmine alum or Nuclear fast red staining and (Brown) DAB staining.