Figure 5.

Stable hESC cell line expressing red fluorescent protein. RNJ9 cells transfected with EF1α-RFP/PGK-Neo were selected with 50 μg/ml G418 for two weeks after transfection and then grown for 10 passages. (a) Fluorescence images of dapi (blue), SSEA4 (green; in fixed cells SSEA4 staining appears punctuate due to the disruption of the cell membrane by paraformaldehyde, and RFP (red). (b) Fluorescence images of dapi (blue), Oct4 (green), and RFP (red).