Research
Therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells after intrathecal administration by lumbar puncture in a rat model of cerebral ischemia
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* Corresponding author: Sin-Soo Jeun ssjeun@catholic.ac.kr
- † Equal contributors
1 Department of Biomedical Science, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Korea
2 Department of Neurosurgery, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, 137-701, Korea
3 Medipost Biomedical Research Institute, MEDIPOST Co., Ltd., Seoul, 137-073, Korea
Stem Cell Research & Therapy 2011, 2:38 doi:10.1186/scrt79
Published: 22 September 2011Additional files
Additional file 1:
Figure S1. Surface antigen characteristic of hUCB-MSCs. Immunophenotyping of hUCB-MSCs. Cells at passage 6 were labeled with antibodies against the indicated antigens and then analyzed by flow cytometry. The results are representative of at least three independent experiments.
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Additional file 2:
Figure S2. Phenotype of transplanted hUCB-MSCs in vivo. Confocal images of the cells at four weeks after 1 × 106 hUCB-MSC administration in the ischemic animal models. hUCB-MSCs were identified by the staining with human nuclei antibody (hNA, green). A small subset of the grafted cells expressed (upper panel) CD73 and (bottom panel) CD105 in the ipsilateral ischemic boundary zone. These markers were immunolabeled with red fluorescence. Nuclei were counterstained with DAPI (blue). Scale bar: 20 μm.
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