Figure 3.

Osteogenic, chondrogenic, adipogenic, and vascular smooth muscular differentiation capacity assessed by staining of BM-derived MSCs cultured in different expansion media. The media are M1 (10% FBS + FGF2), M2 (10% FBS + 5% HPL), M3 (10% HPL), and M4 (5% HPL). Results are of one representative experiment. A, adipogenic differentiation; ASMA, alpha smooth muscle actin; C, chondrogenic differentiation; D0, before differentiation; D14, after 14 days of differentiation; O, osteogenic differentiation; VSM, vascular smooth muscle.