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Resolution: standard / high Figure 4.
High-performance liquid chromatography (HPLC) analysis showing the concentration of
Neu5Gc in cells cultured in xeno-free human embryonic stem cell (hESC) medium (KOSR-XF)
and xeno-containing medium (KOSR). (a) The concentration of Neu5Gc in inactivated and noninactivated NclFed1A cells cultured
in KOSR-XF and KOSR medium for 2 to 6 days. (b, c) The HPLC trace of Neu5Gc concentration in noninactivated NclFed1A cultured for 6 days
in (b) KOSR and (c) KOSR-XF.
Prathalingam et al. Stem Cell Research & Therapy 2012 3:12 doi:10.1186/scrt103 |