High-performance liquid chromatography (HPLC) analysis showing the concentration of Neu5Gc in cells cultured in xeno-free human embryonic stem cell (hESC) medium (KOSR-XF) and xeno-containing medium (KOSR). (a) The concentration of Neu5Gc in inactivated and noninactivated NclFed1A cells cultured in KOSR-XF and KOSR medium for 2 to 6 days. (b, c) The HPLC trace of Neu5Gc concentration in noninactivated NclFed1A cultured for 6 days in (b) KOSR and (c) KOSR-XF.
Prathalingam et al. Stem Cell Research & Therapy 2012 3:12 doi:10.1186/scrt103