Detection of rAAV-mediated transgene expression in undifferentiated monolayer and chondrogenically differentiated aggregate cultures of human mesenchymal stem cells (hMSCs). Cells were transduced with rAAV-lacZ or rAAV-FLAG-hSOX9 (a) in monolayer culture (40 μl each vector) or (b) in aggregate cultures (100 μl each vector), as described in Materials and methods, or left untreated, and processed to monitor transgene expression 21 days after vector application by analyzing the immunoreactivity to the FLAG tag or to SOX9. (a) Anti-FLAG at magnification ×20 and anti-SOX9 at magnification ×4; (b) magnification ×4.
Venkatesan et al. Stem Cell Research & Therapy 2012 3:22 doi:10.1186/scrt113