Histologic analyses in chondrogenically differentiated aggregate cultures of human mesenchymal stem cells (hMSCs). Aggregate cultures were prepared and transduced with rAAV-lacZ or rAAV-FLAG-hSOX9, as described in Figure 1, or left untreated, and processed on day 21 for histologic staining with H&E, toluidine blue, and alizarin red, as described in Materials and methods. All at magnification ×4.
Venkatesan et al. Stem Cell Research & Therapy 2012 3:22 doi:10.1186/scrt113