Table 1 |
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|
Analyses in undifferentiated monolayer cultures of human mesenchymal stem cells (hMSCs) |
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|
Assay |
No vector |
rAAV-lacZ |
rAAV-FLAG-hSOX9 |
|||
|
|
||||||
|
Day 7 |
Day 21 |
Day 7 |
Day 21 |
Day 7 |
Day 21 |
|
|
|
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|
Viable cells |
1,225 (50) |
855 (25)a |
1,250 (45) |
835 (20)a |
1,220 (40) |
840(25)a |
|
Viability (%) |
92 (2) |
53 (2)a |
91 (3) |
51 (2)a |
93 (3) |
53 (2)a |
|
WST -1 (OD450 nm) |
0.34 (0.02) |
0.27 (0.02)a |
0.32 (0.03) |
0.24 (0.01)a |
0.35 (0.03) |
0.26 (0.02)a |
|
DNA (ng/mg total proteins) |
0.19 (0.02) |
0.11 (0.01)a |
0.21 (0.02) |
0.12 (0.01)a |
0.18 (0.01) |
0.14 (0.01)a |
|
|
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|
Values are given as mean (SD) by using a vector dose of 40 μl. aStatistically significant compared with earlier time point. |
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|
Venkatesan et al. Stem Cell Research & Therapy 2012 3:22 doi:10.1186/scrt113 |
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