Table 3 |
||||||
|
Implantation of EOC, HUVEC or MSC does not enhance vascularization, but EOC incorporate into vessels |
||||||
|
Vessel density (Chalkley counts) |
||||||
|
|
||||||
|
Source |
Implanted cells |
Untreated |
Treated |
Difference |
Probability level (ANOVA) |
Incorporation into vessels |
|
|
||||||
|
Umbilical cord blood or normal peripheral blood (donors: n = 5) |
Endothelial outgrowth cells (mice: n = 20) |
7.528 (0.581) |
9.403 (0.671) |
1.875 (0.967) |
0.078 |
Yes |
|
Umbilical vein wall (donors: n = 2) |
Human umbilical vein endothelial cells (mice: n = 7) |
8.238 (1.553) |
8.833 (1.492) |
0.595 (1.502) |
0.706 |
No |
|
Outgrowth from foetal liver MNC (donors: n = 1) |
Endothelial outgrowth cells) (mice: n = 4) |
9.25 (1.975) |
9.25 (0.308) |
0.00 (2.174) |
1.000 |
Yes |
|
Outgrowth from bone marrow MNC (donors: n = 1) |
Mesenchymal stromal cells (MSC) (mice: n = 4) |
5.958 (0.893) |
8.583 (1.788) |
2.625 (2.260) |
0.329 |
No |
|
Umbilical cord blood (donors: n = 1) |
EOC - extended culture (11th passage) (mice: n = 2) |
3.667 (2.000) |
9.333 (2.500) |
5.667 (0.500) |
0.056 |
No |
|
Umbilical vein wall (donors: n = 1) |
HUVEC - early culture (pre first passage) (mice: n = 4) |
3.958 (1.214) |
5.167 (0.649) |
1.208 (1.102) |
0.353 |
Yes |
|
|
||||||
|
Implantation of endothelial outgrowth cells (EOC), human umbilical vein endothelial cells (HUVEC) or mesechymal stromal cells (MSC) did not significantly increase new vessel formation in subcutaneous sponges (P > 0.05), as measured by the difference in vessel density between paired treated sponges containing implanted cells and contralateral control sponges without cells. Vessel density was not different comparing paired cell-impregnated sponges to contralateral control sponges without cells. EOC clearly incorporated into newly formed vessels in all sponges examined, whereas neither HUVEC nor MSC incorporated. Exceptions were late-passage EOC which did not incorporate, or very early HUVEC which did incorporate. Data are means, with standard errors in parentheses. Probability was determined by repeat-sample analysis of variance (ANOVA) on individual readings for each mouse from pooled data from different donors. |
||||||
|
Barclay et al. Stem Cell Research & Therapy 2012 3:23 doi:10.1186/scrt114 |
||||||
