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This article is part of the series Physical influences on stem cells.

Review

Dynamics of branched tissue assembly

Sriram Manivannan1 and Celeste M Nelson12*

Author Affiliations

1 Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA

2 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

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Stem Cell Research & Therapy 2012, 3:42  doi:10.1186/scrt133

The electronic version of this article is the complete one and can be found online at: http://stemcellres.com/content/3/5/42


Published:31 October 2012

© 2012 BioMed Central Ltd

Abstract

The assembly of cells into tissues is a complex process controlled by numerous signaling pathways to ensure the fidelity of the final structure. Tissue assembly is also very dynamic, as exemplified by the formation of branched organs. Here we present two examples of tissue assembly in branched systems that highlight this dynamic nature: formation of the tracheal network in Drosophila melanogaster and the ducts of the mammary gland in mice. Extension of the branches during tracheal development is a stereotyped process that produces identical organ geometries across individuals, whereas elongation of the ducts of the pubertal mammary gland is a non-stereotyped process that produces unique patterns. By studying these two organs, we can begin to understand the dynamic nature of development of other stereotyped and non-stereotyped branching systems, including the lung, kidney, and salivary gland.

Review

The self assembly of cells into tissues and organs is an elegant and intricate process that is vital for development and homeostasis. During organogenesis, the assembly of cells is controlled genetically as well as through cues from cell-cell and cell-matrix interactions [1,2]. Cells assemble into higher-order patterns that are either stereotyped such that there is little variation in the timing and final architecture between individuals, or non-stereotyped, which leads to differences in the details [3]. The branching of the ducts of the pubertal mammary gland is a non-stereotyped process in which the interactions between the ducts and their surrounding microenvironment produce unique tree-like architectures. In contrast, branching of the trachea in Drosophila melanogaster is a stereotyped process that is under strict genetic control. These two systems serve as excellent models with which to investigate the dynamic interplay between cells during tissue formation, under two different modes of control.

Formation of the trachea in D. melanogaster

The trachea of the fruit fly is a ductal structure responsible for the delivery of oxygen to tissues. This organ forms during embryonic development and involves invagination, division, extension and fusion of select cells of placodes along the lateral ectoderm (Figure 1). The determination and positioning of the placodes as well as the choreographed steps that lead to the formation of the trachea is predominantly under the control of the gene trachealess (Trh) [4,5]. At embryonic stage 11, through the activation of the gene rhomboid (Rho) by Trh, the 20 placodes containing approximately 40 cells each invaginate through apical constriction and undergo mitotic division to form 80-cell tracheal sacs [6-8]. The internalized cells then extend from the sites of invagination to form six distinct branches: the dorsal branch (DB), dorsal trunk (DT), visceral branch (VB), lateral trunk (LT), ganglionic branch (GB), and transverse connective (TC). The TC forms from the mid-region of each sac, and DTs extend along the anterior-posterior axis and fuse with DTs of the neighboring sacs to form the main throughway of the trachea. The cells of the DB migrate dorsally and select fusion cells that bind with their counterpart cells of DBs at the opposite side of the embryo [9], while the cells of the LT migrate ventrally and bind with their counterpart LT cells of neighboring metameres to the anterior and posterior, thus fusing the branches and forming additional contiguous pathways for circulation. The cells of the VB and GB do not fuse with those of the neighboring sacs but still branch and extend into the surroundings to complete the tracheal structure [7]. The extensions of the branches are guided by various signaling pathways, including Breathless (Btl) [10], Decapentaplegic (Dpp) [11] and Slit [12]. Even in a strictly stereotyped branching system such as this, however, the role of cellular dynamics is vital and can be seen in the extension and fusion of the DB.

thumbnailFigure 1. Schematic of tracheal development. (a) At embryonic stage 11, the placodes have invaginated and are ready to extend stereotypically. (b) At stage 12, the branches begin extension. (c) At stage 13, the branches have fully extended and begin to fuse. (d) By stage 16, the tracheal fusion is complete. Progenitor cells called tracheoblasts that become activated during metamorphosis remodeling are located in the spiracular branch (SB). (e) Schematic of typical tracheal branch development from embryonic stage 12 to stage 13 to stage 16, after the completion of tracheal branching and fusion and to the completion of metamorphosis remodeling. DB, dorsal branch; DT, dorsal trunk; GB, ganglionic branch; LT, lateral trunk; TC, transverse connective; VB, visceral branch.

The DB consists of approximately six cells and these cells migrate dorsally away from the sac toward the morphogen Branchless (Bnl), which acts as a chemoattractant secreted by the surrounding cells [13]. However, the branch is able to extend normally even when all but one of the cells is devoid of Btl, the receptor for Bnl [14]. The cell with functional Btl receptors acts as a leader and is able to guide the rest of the cells. In wild-type embryos, a leader cell emerges even when every cell expresses Btl. At this stage of development, the cells of the DB are nearly identical and there is no predetermined leader; however, there are small differences in the expression level of Btl. By exploiting these small differences, the cells with the highest expression levels of Btl jockey for the lead position in the newly forming branch, while simultaneously amplifying the difference in the activation of Btl among the population through Notch-mediated lateral inhibition [14-17]. The activation of Btl by Bnl induces the production of Delta, a ligand that binds to and activates Notch in the neighboring cells. Activated Notch inhibits signaling downstream of the Btl receptor and production of Delta within that cell. Over time, this results in one cell with substantially higher Btl activity than its neighbors [16,17]. The successful cell assumes the role of the leader at the tip of the branch while the other cells form the stalk. This establishes a hierarchy and the leader cell is able to efficiently move the branch up the Bnl gradient. As the dorsal branch extends, one of the cells of the branch comes in contact with a cell from the neighboring branch and binds to it, thus fusing the branches to form a contiguous airway [9]. Notch signaling again plays a similar role by aiding Bnl and Dpp and ordaining the fusion cell fate [16,18,19].

During metamorphosis, the trachea once again undergoes significant remodeling. Posterior tracheal branches are lost while anterior branches expand to cover that space and new cells replace most of the cells in the existing branches. Progenitor cells called tracheoblasts that have remained quiescent during embryonic and larval development begin to proliferate and travel along the TC and VB under the guidance of Btl and replace the old cells [20,21]. However, the DBs are replaced not by progenitor cells but by differentiated DB cells that reenter the cell cycle and proliferate to replace the existing branch [21].

Formation of the mammary ducts

The mammary gland is a ductal structure responsible for production and secretion of milk in mammals. The ducts of the mammary gland are composed of a hollow lumen surrounded by a layer of luminal epithelial cells, which are themselves surrounded by a layer of myoepithelial cells that create the basement membrane that separates the epithelium from the stroma. During puberty in rodents, hormones from the ovary and the pituitary gland induce the formation of bulbous terminal end buds (TEBs) at the tips of the rudimentary epithelial ducts [22]. Extension and branching of the TEBs into the surrounding mesenchyme sculpt the highly branched mature mammary epithelial tree. The TEB is composed of multiple layers of preluminal epithelial cells encased in an outer layer of pluripotent stem cells called cap cells (Figure 2) [23]. The cap cells and the preluminal epithelial cells are highly proliferative. The force generated from the rapid proliferation, along with the construction of basement membrane laterally and destruction of extra-cellular matrix at the front, is thought to propel the ducts forward at an average rate of 500 μm per day [24,25]. As the TEBs extend, the cap cells move laterally along the duct and differentiate into myoepithelial cells, while the preluminal epithelial cells give rise to luminal epithelial cells [26]. The structure of the TEB is maintained through E-cadherin- and P-cadherin-containing adherens junctions and netrin-1/neogenin interactions. Preluminal cells interact through E-cadherin, while the cap cells are connected by P-cadherin [27]. Local interactions between netrin-1, a neural guidance cue present on preluminal cells, and neogenin, a netrin receptor present on the cap cells, help maintain the proximity of the preluminal cells to the cap cells [25]. Loss of either netrin or neogenin results in the separation of the cap cell layer from the preluminal layer and destabilization of the TEB [25].

thumbnailFigure 2. Structure of a terminal end bud. The terminal end bud is composed of multiple layers of preluminal epithelial cells encased in an outer layer of pluripotent stem cells called cap cells. The cap cells are connected through P-cadherin (P-Cad) junction, while the preluminal cells are connected through E-cadherin (E-Cad) junctions. Local interactions between netrin-1, a neural guidance cue, present on preluminal cells and neogenin, a netrin receptor, present on the cap cells help maintain the proximity of these two layers.

Estrogen and growth hormone initiate ductal elongation by activating the estrogen receptor (ERα) and the growth hormone receptor, respectively, in the stromal cells surrounding the epithelium [27-29]. The activation of growth hormone receptor enables TEB formation through the insulin-like growth factor pathway [30]. Estrogen signaling leads to the activation of progesterone receptors in the epithelium, which induces epithelial proliferation and ductal movement. Estrogen signaling also stimulates the stromal cells to activate matrix metalloproteinases (MMPs) through amphiregulin and epidermal growth factor receptor [22]. Stromal cells activate several MMPs, including MMP3 and MMP14. MMP14 activates MMP2, which aids in ductal elongation by preventing apoptosis of the cells within the TEB. MMP2 and MMP3 also play a role in determining sites of lateral branching along the duct. High MMP2 prevents the initiation of lateral branches by stimulating the production of transforming growth factor (TGF)β, whereas MMP3 enables lateral branching by degrading the basement membrane and allowing the cells to extend through the fat pad [31].

Unlike tracheal branching, there is no evidence for a global chemoattractant that guides the TEBs through the fatpad [32]. The TEBs weave through the fatpad, extending lateral branches and bifurcating along the way, by integrating the global stimulatory cues with more local cues from neighboring cells, their environment and their own geometry [32]. Patterns of endogenous mechanical stresses arise along the ducts due to asymmetries in their geometry coupled with isometric contraction of the cells and their ability to transmit these forces to their neighbors through adherens junctions [33]. These mechanical stress patterns appear to regulate the sites at which branches initiate. Indeed, three-dimensional organotypic culture models revealed that branches initiate from sites of highest mechanical stress [32], and that cells in these regions of high mechanical stress adopt a more motile mesenchymal phenotype [34,35], increase signaling through focal adhesion kinase (FAK) [32], and increase production of MMPs to aid in degradation of surrounding matrix, all of which enable branch extension [36]. However, while high mechanical stress is necessary for branch initiation, it is not sufficient [32]. Branch initiation and extension are also controlled by the local concentration of morphogens such as TGFβ [37]. High concentrations of TGFβ prevent branching of the ducts, and autocrine secretion of this inhibitory morphogen by the epithelial cells is thought to prevent aberrant branching and maintain proper spacing between ducts [37,38]. By responding to these various stimulatory and inhibitory cues, the TEBs navigate through the stroma until they reach the limits of the fat pad, where they regress and form terminal ducts. However, the ducts still maintain a certain population of undifferentiated stem cells that are capable of self-renewal and repopulation of the entire mammary gland [39,40]. These undifferentiated cells are thought to aid in the remodeling of the mammary gland during and after pregnancy, where further ductal expansion and regression occur, respectively [40].

Conclusion

Branching of the trachea and the mammary gland high-light the dynamic process of tissue formation. These two systems show how stem cells, progenitor cells and differentiated cells come together, and through a combination of global and local guidance cues specify the final architecture of the tissue. During puberty in mammals, the pluripotent cap cells combined with other cell types of the TEB and through the regulation of the micro-environment produce the unique pattern of the ductal tree. In the formation of D. melanogaster trachea, even though the final architecture is fixed, the cells of the invaginated tracheal placodes have a high degree of freedom to determine the leaders, the fusion cells and those that will remain quiescent during the various branch formations. In addition to normal development, the dynamics of stem cells also come into play during tumor formation. Cancer stem cells (CSCs) are specialized cells within tumors that are capable of self-renewal and initiation of tumors [41]. CSCs have all the characteristics of normal stem cells and are even capable of generating non-malignant structures [42,43]. The origin of CSCs is still hotly debated. While the similarities between CSCs and normal stem cells might strongly suggest that CSCs arise through aberrant differentiation of normal stem cells, there is also evidence to suggest that CSCs might arise from progenitors or even through dedifferentiation of fully differentiated cells. Understanding the dynamics of cells during normal development will help in understanding CSCs and malignant development in general, as many of the processes involved in normal development are conserved in malignant development.

Thanks in large part to advances in technology, we are finally able to glimpse at the complexities of developmental processes. However, better in vivo imaging approaches are needed to fully answer most of the open questions, as it is near impossible to investigate dynamic processes using still images. Advances in live imaging could help, for example, to study the formation and movement of the TEBs. Studies in culture have suggested that organization of the TEBs might involve cellular sorting to optimize the structure for invasion through the fatpad [44]; however, this has yet to be shown in vivo. Furthermore, the mechanism by which the TEB moves through the fatpad is still unclear [45]. In addition to imaging, advances in techniques to manipulate in vivo microenvironments might prove to be the most fruitful venture. The mechanics of the microenvironment integrate with molecular cues in the regulation of cell division, motility, branching, and stem cell differentiation [46]. Laser ablation studies in D. melagonaster have been used to show the involvement of tensile forces in tracheal branching [47-49]. At present, however, culture models still provide one of the best means of studying the effects of the mechanics of the microenvironment on these processes in a controlled and quantifiable manner. While they do suffer from drawbacks of artificiality, sophisticated three-dimensional culture models can aid to bridge the gap between the plastic world and the in vivo world, and could play an important role in studying the interplay between multiple cell types during development [50,51]. A better appreciation of the dynamic nature of tissue assembly will have a great impact on the study of normal and malignant development and may also will help advance the field of tissue engineering.

Note

This article is part of a thematic series on Physical influences on stem cells edited by Gordana Vunjak-Novakovic. Other articles in the series can be found online at http://stemcellres.com/series/physical webcite

Abbreviations

BNL: Branchless; BTL: Breathless; CSC: cancer stem cell; DB: dorsal branch; DPP: Decapentaplegic; DT: dorsal trunk; GB: ganglionic branch; LT: lateral trunk; MMP: matrix metalloproteinase; TC: transverse connective; TEB: terminal end bud; TGF: transforming growth factor; Trh: Trachealess; VB: visceral branch.

Competing interests

The authors declare that they have no competing interests.

Acknowledgements

Work from the authors' lab was supported in part by the NIH (CA128660, GM083997, and HL110335), Susan G Komen for the Cure, the David and Lucile Packard Foundation, and the Alfred P Sloan Foundation. CMN holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund.

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